为安全有效地防控烟草靶斑病,采用RNA干扰技术,以烟草靶斑病菌(Rhizoctonia solani YC-9)的内切多聚半乳糖醛酸酶(endoPGs)和MAP激酶为靶点,设计并合成dsRNA-endoPGs和dsRNA-RMK1,对烟草离体叶片和大田烟株喷施dsRNA-endoPGs和dsRNA-RMK1并分析其对烟草靶斑病的防治效果.结果表明,将dsRNA-endoPGs和dsRNA-RMK1喷施于烟草离体叶片表面,7 d后烟草靶斑病的病斑抑制率分别为62.10%和60.00%;向大田烟草叶片表面分别喷施dsRNA-endoPGs和dsRNA-RMK1,每7d喷施1次,共喷施3次,首次施药后7、14、21 d的烟草靶斑病相对防效均高于50%.
To safely and effectively prevent and control tobacco target spot disease,endo polygalacturonases(endoPGs)and MAP kinases of the tobacco target spot pathogen(Rhizoctonia solani YC-9)were used as the targets to design and synthesize dsRNA-endoPGs and dsRNA-RMK1 by RNA interference technology.The control effects of dsRNA-endoPGs and dsRNA-RMK1 on tobacco target spot were analyzed by spraying on tobacco leaves in vitro and tobacco plants in the field.The results showed that 7 d after spraying dsRNA-endoPGs and dsRNA-RMK1 on tobacco leaves in vitro,the inhibition rates of tobacco target spot lesions were 62.10%and 60.00%,respectively.After spraying dsRNA-endoPGs or dsRNA-RMK1 on the leaves of tobacco plants in field trials once every 7 d and 3 times in total,the relative control efficacies on tobacco target spot 7,14,21 d after the first spray were all above 50%.