目的:建立慢性非细菌性前列腺炎(CNP)并肝郁大鼠模型的方法,探讨肝郁与CNP的关系。方法采用Wistar雄性大鼠50只,模型组为37只,前列腺蛋白制备组5只,空白组8只。其中模型组随机选取6只作为CNP组,6只作为肝郁并CNP组。先采用弗氏免疫佐剂法制备CNP模型,观察前列腺病理变化和肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)浓度变化,然后对模型组采用束缚法制备肝郁并CNP模型,观察前列腺病理变化和TNF-α、IL-1β浓度变化。结果采用弗氏免疫佐剂法能导致大鼠前列腺出现炎性改变和TNF-α、IL-1β浓度升高。CNP组和肝郁并CNP组大鼠前列腺组织TNF-α和IL-1β浓度均高于空白组,差异有统计学意义(P<0.05);肝郁并CNP组大鼠前列腺组织TNF-α和IL-1β浓度均高于CNP组,差异有统计学意义(P<0.05)。结论弗氏免疫佐剂法结合束缚法能制备肝郁并CNP模型,肝郁能加重CNP的炎性改变。
Objective To establish method of chronic non-bacterial prostatitis (CNP) and liver depression rat model and probe into the relationship between liver depression and CNP. Methods 50 male Wistar rats were chosen for ex-periment, among which 37 rats were chosen for model group, 5 rats were chosen for preparation group and the other 8 rats were chosen for blank group. 6 rats were randomly selected as CNP group and 6 rats as liver depression combined with CNP group in model group. First, the model of CNP were made by Freund’s adjuvant method to observe the patho-logical change of prostate and level change of TNF-α, IL-1β; then, the liver depression pattern of CNP was made by binding method to observe the pathological changes of prostate and level change of TNF-α, IL-1β. Results Freund’s adjuvant method could lead to rat prostate inflammatory change and the level rising of TNF-α and IL-1β. TNF-α, IL-1β level of rat prostate in CNP group and liver depression combined with CNP group was higher than that in blank group respectively, with statistical difference (P< 0.05). TNF-α, IL-1β level of rat prostate in liver depression com-bined with CNP group was higher than that in CNP group respectively, with statistical difference (P< 0.05). Conclu-sion Freund’s adjuvant method combined with the binding method can prepare liver depression pattern of CNP, liver depression can aggravate inflammatory change of CNP.