建立同时测定肉类食品中尿酸、鸟嘌呤、次黄嘌呤、黄嘌呤和腺嘌呤的高效液相色谱分析方法,并测定肉类样品中上述4种嘌呤及尿酸的含量.色谱条件:资生堂CAPCELL PAK-C18(4.6 mm×250 mm,5μm)色谱柱,柱温30℃;流动相为7×10-3 mol/L KH2PO4-H3PO4(pH =3.83),流速1.0 mL/min;检测器为紫外检测器,检测波长254 nm;进样量10 μL.结果:在上述条件下4种嘌呤和尿酸分离和测定效果良好,4种嘌呤和尿酸的质量浓度和峰面积在0.05 ~ 50 μg/mL线性范围内线性关系良好,相关系数均在0.999 6以上,检出限在0.010~0.024 μg/mL之间,精密度检测RSD为0.25% ~0.81%,各组分回收率为92.0%~105.0%,方法精密度RSD为6.27% ~11.09%.结论:该方法简便,快捷,可靠,各组分分离度好,可应用于肉类食品中嘌呤和尿酸的同时分析测定.
A high performance liquid Chromatography method for the determination of uric acid,guanine,hypoxanthine,xanthine and adenine in meat was established and applied.The detecting method for Chromatography was:Shiseido CAPCELL PAK-C18 (4.6 mm × 250 mm,5 μm) column,column temperature was at 30 ℃.The mobile phase was 7 × 10-3 mol/L KH2PO4-H3PO4 (pH =3.83)at a flow rate of 1.0 mL/min.The UV detector was used and the wavelength was set at 254 nm and the Sample volume was 10 μL.Result:Under the above conditions,good separation was obtained and four kinds of purines and uric acid was showed in the range of 0.05-50 μg/mL;a good linear relation was observed between the concentration and peak area,and all the correlation coefficients are higher than 0.9996,the limit of detection was between 0.010-0.024 g/mL,precision detection of RSD% was between 0.25%-0.81%,the recovery rate of each component was between 92.0%-105.0%,the precision RSD% was between6.27%-11.09%.Conclusion:The method was simple,rapid and reliable,had a good separation for each component,and can be used for the analysis and determination of purine and uric acid in meat products.