Amphichoterpenoids A–C (1–3), unprecedented picoline-derived meroterpenoids possessing a pyrano [3,2-c]pyridinyl-γ-pyranone scaffold, were characterized from the ascidian-derived fungus Amphichorda felina SYSU-MS7908. Their structures were elucidated by spectroscopic methods, X-ray diffraction and electronic circular dichroism (ECD) calculations. A plausible biosynthetic pathway was proposed. The isolated compounds displayed moderate inhibitory activity against acetylcholinesterase with 50%inhibiting concentration (IC50) values of 18.8–53.2μmol/L.