以杉木林下草珊瑚为研究对象,通过测定其根系中丛枝菌根真菌(AMF)侵染率、根际土壤菌丝密度、根内AMF 群落多样性以及土壤理化性质,探讨草珊瑚根内 AMF 群落结构的季节动态,以及与土壤理化性质的关系.结果表明,在草珊瑚根系中共检测到 440 个 AMF 的分类单元,分属 9 科 14 属,其中球囊霉属为绝对优势属,且 1 月和 9月相对丰度高达 99%.AMF侵染率和菌丝密度在不同月份存在波动,侵染率也是在 1 月和 9 月有两个峰值,且 9 月根际土壤的菌丝密度最大.土壤全磷、全钾、有效磷、速效钾和含水量是影响草珊瑚根系AMF群落结构的主要因子,其中土壤全磷和含水量影响显著(P<0.05).
This study documented the seasonal variation of arbuscular mycorrhizal fungal(AMF)community associated with Sarcandra glabra planted under Cunninghamia lanceolata forest stand.By quantifying the mycorrhizal colonization rate,fungal hyphal density in rhizosphere soil,diversity of AMF,as well as the rhizosphere soil properties,we analyzed the potential effects of soil properties on AMF community structure's seasonal dynamics.We identified 440 OTUs in the roots of S.glabra,including 14 genera and 9 families of AMF.Glomus was the dominant genus.The relative abundance of Glomus was highest in January and September,reaching up to 99%.Meanwhile,the mycorrhizal colonization was higher in January and September than in other months,and the hyphal density in the rhizosphere soil was the highest in September.Soil total phosphorus,total potassium,available phosphorus,available potassium,and soil water content were the main factors affecting the structure of AMF community in roots of S.glabra,with the effects of total phosphorus and water content being significant(P<0.05).