颅内感染是神经外科常见疾病之一,也是神经外科术后严重并发症之一,其进展是由所感染微生物与宿主相互作用所产生.治愈颅内感染的关键问题是尽早、准确地识别感染病原体,并精准使用病原菌有效的抗生素.而仅依靠传统检测方法识别导致颅内感染的病原体并非易事.近年来二代测序技术(NGS)的迅速发展,使早期确认感染病原体成为可能,此技术可以高通量、快速高效、高精度的检测病原体,同时还能够为临床用药提供指导.NGS作为一种新的病原检测手段,自问世以来就受到了极大的关注,本文将其在不同病原体所致颅内感染的应用做综述,以期让广大临床工作者充分了解其优势及劣势,使NGS技术能够在诊断颅内感染中发挥更大作用,从而减轻患者痛苦.
Intracranial infection is one of the most common neurosurgical diseases and one of the most serious postoperative complications of neurosurgery.They progress as a result of the interaction of the infected microorganisms with the host.The key issues in curing intracranial infections are early and accurate identification of the infecting pathogen and precise use of pathogenically effective antibiotics.Identifying the pathogens responsible for intracranial infections is not an easy task when relying only on traditional testing methods.In recent years,the rapid development of second-generation sequencing technology(NGS)has made it possible to identify infectious pathogens at an early stage,and this technology can detect pathogens in a high-throughput,rapid,and efficient manner with high precision while also providing guidance for clinical drug use.NGS,as a new mean of detecting pathogens,has attracted a great deal of attention since its inception,and this article provides an overview of its application to intracranial infections caused by different pathogens,in order to let clinicians fully understand its advantages and disadvantages,so that NGS technology can play a greater role in the diagnosis of intracranial infections,thus alleviating the pain of patients.