目的 探讨细胞因子白细胞介素( 1L)-8(- 251)位点及其受体CXCR2(±1208)位点多态性与乳腺癌发病风险及侵袭特性的关系.方法 采用等位基因特异-聚合酶链反应(AS-PCR)分析方法检测228名乳腺癌患者和100例健康对照者的IL-8(- 251)位点及其受体CXCR2(±1208)位点多态性分布,并进行统计学分析.结果 IL-8(- 251)位点TA型和AA型以及CXCR2(± 1208) TT型是乳腺癌的高危基因型[比值比(OR)=1.57,95%可信区间(CI)=1.08~2.32;OR =2.68,95% CI=1.26 ~2.99;0R=2.02,95%CI=1.08~3.62],在两组间表达频率的差异有统计学意义(P<0.05).携带1个及以上高危基因型增加患乳腺癌的风险.分层分析表明携带IL-8(-251)等位基因A和CXCR2(± 1208)等位基因T增加乳腺癌的侵袭特性,其分布频率在肿瘤的高组织学分级(OR=1.95,P<0.01;OR=1.52,P<0.05)和淋巴结转移阳性(OR=1.65,P<0.05;0R=1.66,P<0.01)中差异有统计学意义.另外,IL-8(-251)A等位基因与ER( -)乳腺癌显著相关( OR=1.65,P<0.05).结论 IL-8和CXCR2的基因多态性可能与女性乳腺癌的发生发展关系密切,可作为乳腺癌早期基因诊断的指标.
Objective To explore the association between interleukin (IL)-8 gene -251 A/T and CXCR2 gene ± 1208 C/T polymorphisms with the risk and aggressive forms of breast cancer.Methods Totally 228 breast cancer patients and 100 non-cancer female controls were selected.The genotypes were detected by using AS-PCR method.Results IL-8 ( -251 ) TA/AA and CXCR2 ( ± 1208 ) TT were the high risk genotypes ( OR =1.57,95% CI =1.08-2.32 ; OR =2.68,95% CI =1.26-2.99 ; OR =2.02,95% CI =1.08-3.62 ),and there was significant difference in the expression distributation between the case group and the control group.The risk of breast carcinoma was significantly in the patients carrying one high-risk genotype and above7.The stratified analysis indicated that IL-8 ( - 251 ) A allele and CXCR2 ( ± 1208) T allele are highly associated with aggressive forms of breast cancer as denied by high grade and lymph node metastases (OR =1.65,95% CI =1.21-2.74; OR =1.66,95% CI =1.17-2.73 ).Conclusion The results indicated that the polymorphisms in IL-8 and CXCR2 genes,which might be associated with breast cancer risk of Chinese women,as well as disease progress,could be used as an indicator of the early genetic diagnosis of breast cancer.