目的 探讨慢乙肝聚乙二醇干扰素治疗疗效与TP区和RT区复杂程度的相关性.方法 入组HBeAg阳性,HBV DNA阳性的慢乙肝患者,给予聚乙二醇干扰素180 μg每周1次皮下注射治疗,收集基线患者血清和患者基线与治疗12周临床信息.对基线HBV DNA的TP区和RT区片段进行扩增、建库、高通量测序,计算香农商和平均遗传距离.结果 对108例患者进行logistic回归分析.RT区片段马氏距离和TP区片段的香农商有利于HBV DNA应答,ALT有利于HBeAg应答,HBsAg不利于HBsAg应答.结论 基线TP区和RT区核酸可能与慢乙肝聚乙二醇干扰素治疗疗效有关.
Objective To explore the association between the efficacy of peg-IFN and the complexity of TP and RT regions of hepatitis B virus (HBV) in chronic hepatitis B.Methods Patients with HBeAg positive,HBV DNA positive chronic hepatitis B were given peg-interferon 180 μg once a week for subcutaneous injection,and baseline information was collected from baseline and after 12 weeks' treatment.The baseline HBV DNA TP and RT fragments were amplified,database,high-throughput sequencing,and the average genetic distance calculation.Results Data of 108 patients were analyzed by logistic regression.RT area fragment Markov distance and TP area fragment Shannon quotient for HBV DNA response were calculated.ALT level is good for HBeAg response.HBsAg level is bad for HBsAg response.Conclusions The complexity of the baseline TP and RT regions may be associated with the efficacy of peg-interferon therapy for CHB.