为了解不同芥菜型油菜种质资源的耐盐程度,本研究利用0、150、200和250 mmol/L浓度的NaCl溶液对479份来自不同国家的芥菜型油菜种质资源进行盐胁迫处理,并测定发芽率、根长、芽长和鲜重;采用主成分分析、隶属函数、多元线性回归分析和聚类分析对参试材料的耐盐性进行综合评价.结果发现在200 mmol/L的NaCl溶液处理下,可以有效地将479份材料划分为4类,第I类为耐盐品种12份,第Ⅱ类为75份中等耐盐品种,第Ⅲ类为313份不耐盐品种,第Ⅳ类为79份盐极敏感品种,并建立了芥菜型油菜耐盐性鉴定的多元线性回归方程,y=0.537x1+ 0.310x2+0.089x3+0.064x4.筛选得到的耐盐性材料为培育油菜耐盐新品种奠定基础.
To understand the salt tolerance of different Brassica juncea germplasm resources,this study used Na-Cl solutions with concentrations of 0,150 mmol/L,200 mmol/L,and 250 mmol/L to treat 479 B.juncea germplasm re-sources from different countries under salt stress,and measured germination rate,root length,bud length,and fresh weight;Comprehensive evaluation of the salt tolerance of the test materials was conducted using principal component analysis,membership function,multiple linear regression analysis,and cluster analysis.The results showed that under the treatment of 200 mmol/L NaCl solution,479 materials could be effectively classified into 4 categories:(1)12 salt tolerant varieties;(2)75 moderate salt tolerant varieties;(3)313 salt tolerant varieties;and(4)79 salt sensitive variet-ies.A multiple linear regression equation was established for the identification of salt tolerance in B.juncea,y= 0.537x1+0.310x2+0.089x3+0.064x4.The selected salt tolerant materials lay the foundation for cultivating new salt tol-erant B.juncea.