为研究地皮菜不同极性萃取部位活性成分含量及其抗氧化与体外抑菌作用,采用超声辅助提取法提取后得到石油醚相、乙酸乙酯相、正丁醇相和水相4个极性萃取部位.测定4个不同极性萃取部位总三萜、总黄酮和总多酚得率,以维生素C(VC)为对照,测定不同极性萃取部位对ABTS、DPPH、O2-自由基的清除能力和还原能力;用滤纸片法测定其抑菌圈大小并分析其抑菌活性.结果表明,地皮菜乙酸乙酯相总三萜、总黄酮和总多酚得率分别为0.33%、2.40%、0.67%.各极性萃取部位均有一定的抗氧化能力及抑菌活性,其中乙酸乙酯相提取物的抗氧化能力及抑菌活性最好.这为今后利用地皮菜中生物活性物质和开发地皮菜抗氧化产品提供一定的理论依据.
In order to study the active components contents in different polar extracts of Nostoc commune and their antioxidant and antibacterial ef-fects in vitro,4 polar extracts including petroleum ether phase,ethyl acetate phase,n-butanol phase and water phase were obtained by ultrasonic-as-sisted extraction method.The contents of total triterpenes,total flavonoids,and total polyphenols in 4 different polar extracts were determined.Using vitamin C(VC)as the control,the radical scavenging of different polar extracts on ABTS+,DPPH,O2 free radical,and reducing power were determined.The inhibition zone size and its antibacterial activity were measured by filter paper method.The results showed that the contents of total triterpenes,total flavonoids and total polyphenols in ethyl acetate phase of N.commune were 0.33%,2.40%and 0.67%,respectively.All polar extracts had cer-tain antioxidant activity and antibacterial activity.Among them,the extract of ethyl acetate phase had the optimal antioxidant capacity and antibacte-rial activity.The study provided a theoretical basis for the utilization of bioactive substances in N.commune and the development of antioxidant products of N.commune.