目的 探讨天智颗粒对帕金森病(PD)小鼠模型脑黑质和脊髓多巴胺(DA)能神经元的影响.方法 2022年9月采用1-甲基-4-苯基-1,2,3,6-四氢吡啶25 mg/(kg·d)腹腔注射5 d的方法构建PD小鼠模型,采用随机数字表法分为模型组和天智颗粒5、2、1 g/kg组,每组10只,另取10只小鼠作为正常对照组.灌胃给药2周后比较各组小鼠行为学变化、中脑黑质和脊髓DA能神经元存活数量及形态变化、神经元内酪氨酸羟化酶阳性表达情况等.结果 与正常对照组比较,模型组小鼠悬挂能力下降、游泳时间缩短,经天智颗粒干预后可有效提高小鼠悬挂能力,延长游泳时间,黑质和脊髓灰质前角DA能神经元数量和神经元内酪氨酸羟化酶阳性表达均明显减少,经天智颗粒5、2 g/kg干预后小鼠黑质和脊髓DA能神经元检测指标明显改善,差异均有统计学意义(P<0.05).结论 天智颗粒可改善PD小鼠行为学,提高中脑黑质和脊髓部位DA能神经元及突起的存活数目,增加中枢神经系统内DA的含量,表明天智颗粒对黑质和脊髓灰质前角多巴能神经元具有保护作用.
Objective To investigate the effect of Tianzhi Granule on dopaminergic(DA)neurons in substantia nigra and spinal cord of Parkinson's disease(PD)mouse model.Methods PD mouse model was constructed by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine 25 mg/(kg·d)for five days in September 2022.The mice were randomly divided into the model group and the Tianzhi Granule 5,2,1 g/kg groups,with 10 mice in each group.Another 10 mice were taken as the normal control group.Af-ter two weeks of intragastric administration,the behavioral changes of mice in each group,the number and morphology changes of DA neurons in the substantia nigra and spinal cord,and the positive expression of tyro-sine hydroxylase in neurons were compared.Results Compared with the normal control group,the suspension ability of mice in the model group was decreased and the swimming time was shortened.After the intervention of Tianzhi Granule,the suspension ability of mice was effectively improved,the swimming time was pro-longed.The number of DA neurons in the anterior horn of the substantia nigra and spinal cord gray matter and the positive expression of tyrosine hydroxylase in neurons were significantly reduced.After five and two g/kg intervention with Tianzhi Granule,the detection indexes of DA neurons in the substantia nigra and spinal cord of mice were significantly improved,and the differences were statistically significant(P<0.05).Conclusion Tianzhi granule can improve the behavior of PD mice,increase the survival number of DA neurons and neurites in the substan-tia nigra and spinal cord,and increase the content of DA in the central nervous system,indicating that Tianzhi granule has a protective effect on dopaminergic neurons in the substantia nigra and spinal cord.