系统介绍绿藻在厌氧条件下利用氢化酶产氢的3种不同途径,包括依赖光系统Ⅱ的直接产氢、不依赖光系统Ⅱ的间接产氢和暗发酵产氢,同时分析3种产氢途径各自的优缺点.受技术和制造成本限制,绿藻产氢仍处于初步开发阶段,实现大规模工业生产仍需很长一段时间.针对提高绿藻产氢效率、实现工业化的目标,综述该领域最新研究结果,概括从氢化酶改造、降低体系中氧气含量、减弱环式电子传递、降低卡尔文循环、缩小捕光天线、底物预处理和光生物反应器设计等7个方向优化产氢反应的方式,并展望绿藻产氢的未来发展趋势.
This review systematically introduced three different pathways of hydrogen production by hydrogenase in green algae under anaerobic condition,including direct hydrogen production via photosystem Ⅱ,indirect hydrogen production independent of photosystemⅡ and hydrogen production by dark fermentation.The advantages and disadvantages of each pathway were analyzed.However,limited by technology and high costs,hydrogen production by green algae was still in the laboratory-scale,and will take a long time to achieve industrial production.Aiming at the efficient hydrogen production,this review described the latest outcome in this field and summarized the methods for optimizing the hydrogen production,including hydrogenase modification,reducing the oxygen content,lowering the cy-clic electron transfer rate,reducing the Calvin-cycle,remodeling the light harvesting complex,pretreatment of the biomass and photo-bioreactor design.The future development of hydrogen production by green algae was also discussed.