PIN2蛋白在水稻根尖细胞中定位方法的优化 / Optimization of Localization Methods for PIN2 Protein in Cells of Root Tips of Rice
- Resource Type
- Academic Journal
- Authors
- 李琰; 詹晓平; 杨静; 刘林; 李成云; Li Yan; Zhan Xiaoping; Yang Jing; Liu Lin; Li Chengyun
- Source
- 山东农业科学 / Shandong Agricultural Sciences. (8):104-106
- Subject
- PIN2 蛋白
免疫荧光定位
水稻
根尖细胞
PIN2 protein
Immunolocalization
Rice
Cells of root tips
- Language
- Chinese
- ISSN
- 1001-4942
PIN2蛋白作为生长素外排蛋白,在植物根的向地性生长中起重要作用。免疫定位法是一种可以将蛋白在植物细胞中快速准确定位的方法。本研究利用改良的免疫荧光定位法,对 PIN2蛋白在水稻根尖细胞中的定位进行了观察,并与前人的两种方法进行比较。结果表明,与已有的方案比较,改良免疫荧光定位法可使 PIN2蛋白在水稻根尖细胞中的位置快速准确地被观察到,且简化了操作步骤,操作方便快速,可广泛应用于植物其他蛋白的定位研究。
PIN2 as an auxin efflux protein plays important roles in geotropic growth of plant roots.Immu-nolocalization technique is a method which allows rapid and reliable in situ localization of proteins in plant cells.In this paper,the improved immunolocalization technique was used to localize the PIN2 in root tip cells of rice,and its effect was compared to the two existing methods.The results showed that the location of PIN2 could be more quickly and accurately determined in root tip cells of rice by the improved method;and the im-proved method simplified the operation procedures and was more convenient,so it could be used for the locali-zation researches of other plant proteins in the future.