溴氰菊酯( deltamethrin,DM)是一种神经毒剂,由于其高效、低毒而被广泛用于农业害虫的防治。长期大范围不合理的使用导致害虫产生抗药性,但目前其确切的抗药性作用机制仍不十分清楚。本研究对溴氰菊酯细胞毒性的研究显示DM染毒可以使果蝇Kc细胞增殖、细胞形态和细胞活性发生明显变化。较低和较高浓度的DM能明显抑制细胞活性,在15 ppm诱导下果蝇细胞的活性有一定程度的升高。实时荧光定量PCR和免疫印迹分析结果显示,经DM处理后的果蝇Kc细胞中与氧化应激反应和免疫相关的基因的表达量显著上调。本研究对细胞毒性和基因表达差异的检测,为进一步探讨与溴氰菊酯抗药性相关的细胞和分子毒理提供了研究基础。
Deltamethrin( DM) is a kind of nerve agent,and commonly used in controlling crop pests,because of the high efficacy,low toxicity. Widespread and improper use of DM induced insect resistance,but the resistance mechanism is not clear at present. Cytotoxicity analysis reveals that the cells proliferation, cellular morphology and cells viability have significant changes under DM stress. Too Low or too high concentration of DM could obviously inhibit the activity of cells. Under 15 ppm treatment, cell activity was higher. Real-time quantitative PCR and western-blotting analysis demonstrated that the genes associated with oxidative stress and immune responses were induced sharply under DM stress. Our research focuses on the cytotoxicity and gene differential expression,these results can provide basis for the study of molecular toxicology and mechanism of deltamethrin resistance.