为探究星孢寄生菇在光胁迫下的生长发育特性,采用平板培养法,研究了 10 种不同光质LED光源对该菇菌丝生长、原基分化及子实体发育的影响.结果表明,与黑暗对照相比,不同光源对该菇菌丝生长均有抑制作用,其中白光的抑制效果最显著.同时,光源对该菇原基分化数量的影响不显著,但白光培养下的原基分化时间最长(3.75 d).此外,暖黄光对该菇子实体发育有促进作用,蓝光有抑制作用,紫光有先抑制后促进的作用.接种后2~3 d,该菇菌丝生长速度和原基分化数量呈线性增长趋势,在大多数光源下分别在第 3、4 天达到峰值;第 3~4 天,在大多数光源下,该菇子实体发育数量也呈线性增长趋势,并达到峰值.值得注意的是,在某些光源下,该菇菌丝生长速度、原基分化数量和子实体发育数量在高峰期后仍呈现小高峰.总之,光胁迫对该菇菌丝生长不利,对原基分化影响较小,但对子实体发育有正负两方面的影响.
In order to investigate the growth and development characteristics of Asterophora lycoperdoides under light stress,the effects of 10 different light quality LED light sources on the mycelium growth,primordium differentiation and fruiting body development of this mushroom were investigated by plate culture method.The results showed that different light sources inhibited the mycelial growth of A.lycoperdoides,with white light showing the most significant inhibitory effect.Meanwhile,the effect of light source on the number of its primordium differentiation was not significant,but the longest time of primordium differentiation(3.75 d)was observed under white light culture.In addition,warm yellow light promoted the development of its fruiting body development,while blue light had an inhibitory effect,and violet light tended to inhibit and then promote the development of this mushroom.From 2 to 3 d after inoculation,the mycelium growth rate and the number of primordium differentiation of A.lycoperdoides showed a linear increasing trend,and reached the peaks on the 3 d,4 d under most of the light sources,respectively;from 3 to 4 d,under most light sources,the number of its fruiting body development also showed a linear growth trend and reached a peak.It is worth noting that the mycelium growth rate,the number of primordium differentiation and the number of fruiting body development of this mushroom still showed small peaks after the peak period under some light sources.In conclusion,light stress was detrimental to the mycelium growth of A.lycoperdoides and had a minor effect on the primordium differentiation,but had a positive and negative effect on the development of the fruiting body.