以厌氧序批式生物膜反应器(AnSBBR)处理葡萄酒生产废水,考察反应器在持续负荷冲击下的运行特性及群落结构响应.结果显示,持续负荷冲击的前13d(29~41d),反应器运行稳定;因逐渐增加的氢分压和挥发性脂肪酸(VFAs),到56d时系统稳定性降低(VFA/TA=0.72),乙酸比产甲烷活性(SMA)降低46.2%,但氢利用速率(HUR)增加69.2%,辅酶F420浓度增加11.9%.在42d时仅外层的Slime-EPS浓度明显增加(34.1%);在56d时TB-EPS和LB-EPS浓度增加61.3%和62.8%,其PN/PS比值增加197.8%和126.0%,负荷冲击诱导EPS分泌大量的应急性蛋白类产物,其电活性物质提高了系统的电子传递活性(35.5%).Illumina MiSeq 显示,负荷冲击下 Desulfovibrio、Ruminococcus 和Geobacter等产酸菌丰度降低,而 Methanobacterium 丰度由 32.2%增至 50.9%.生物膜系统通过逐级EPS分泌和强化还原CO2产甲烷来响应持续负荷冲击的影响.
A laboratory-scale anaerobic sequencing biofilm batch reactor(AnSBBR)was used for the digestion of winery wastewater,and its operational characteristics and microbial community structure under continuous shock load were investigated.The results showed the AnSBBR maintained high removal efficiency and stability during the first 13days(29~41d)of continuous shock load.With the increase of hydrogen partial pressure and VFAs contents,the stability decreased(VFA/TA=0.72),SMA decreased by 46.2%,HUR increased by 69.2%,and coenzyme F420concentration increased by 11.9%.Compared with the steady stage,only the Slime-EPS contents increased significantly(34.1%)at day 42,while at day 56,the TB-EPS and LB-EPS contents increased by 61.3%and 62.8%,respectively,and the PN/PS ratio increased by 197.8%and 126.0%.Continuous shock load induced EPS to release massive emergency protein products to resist stress,and the electroactive substance enhanced the electron transfer activity(35.5%).Illumina MiSeq has shown that the abundance of acidogenic bacteria such as Desulfovibrio,Ruminococcus,and Geobacter decreased,while the abundance of Methanobacterium increased from 32.2%to 50.9%under continuous shock load.The biofilm system responds to the impacts of continuous shock load by EPS layered secretion and enhanced the methanogenic pathway utilizing CO2 and H2.