为了解西南部分地区非人灵长类贾第虫的感染情况及其集聚体和基因亚型分布,收集了西南地区部分动物园、养殖场以及试验动物养殖基地的207份猕猴、长臂猿、金丝猴和食蟹猴新鲜粪便,采用饱和蔗糖漂浮法处理样品,提取DNA,经巢式PCR扩增β-giardin(bg)、tpi和gdh基因,扩增产物经测序后进行分子生物学分析.结果表明:西南部分地区圈养非人灵长类贾第虫感染率为7.73%(16/207),16份阳性样品均为集聚体B(assemblage B).感染的品种包括猕猴、长臂猿及食蟹猴.长臂猿感染率最高(38.89%),不同品种非人灵长类贾第虫感染率差异极显著(P<0.01).所有阳性样品均成功扩增出bg、tpi和gdh三个基因的特异性产物.多位点基因序列分析和种系进化树分析结果显示,bg及tpi基因位点多态性变异明显,gdh位点多态性变异较小.本次调查结果表明,西南部分地区非人灵长类所携带的贾第虫具有人兽共患风险.
To understand the prevalence and molecular characteristics of Giardia duodenalis in captive non-human primates(NHPs)in parts of Southwest China, a total of 207 fresh fecal samples from rhesus macaques, northern white-cheeked gibbons,golden snub-nosed monkeys and crab-eating macaques were obtained from zoos, farms and laboratory in Southwest China. The oocysts in samples were collected by the Sheather's sucrose flotation technique,and their DNA was extracted. These DNA samples were used to amplify β-giardin(bg),tpi and gdh genes by nest PCR for detecting Giardia. The results showed that 7.73%(16/207)of samples were positive for G. duodenalis in-fection,and 16 positive samples from rhesus macaques, northern white-cheeked gibbons and crab-eating macaques were identified as assemblage B. Northern white-cheeked gibbons had the highest infection rate(38.89%),and the difference in infection rates among the species was significant(P<0.01). All the positive samples were successfully amplified based on bg, tpi and gdh genes, multilocus genotyping showed the high genetic diversity of bg and tpi. Based on a phylogenetic analysis, potentially zoonotic genotypes of Giardia duodenalis were identified in NHPs in southwest China.