目的 探讨葛根素对子痫前期(PE)大鼠模型胎盘氧化应激及免疫状态的影响.方法 将45只雌性SD孕鼠随机分为对照组,模型组,实验组,每组15只.模型组和实验组大鼠于孕13~21 d皮下注射L-精氨酸甲酯(L-NAME)100 mg·kg-1,qd,对照组注射等量0.9% NaCl,连续给药至孕21 d;孕17~21 d,实验组腹腔注射葛根素溶液80 mg·kg-1,qd,对照组及模型组注射等量0.9% NaCl,连续给药至孕21 d.分别于孕10,16,21 d检测各组大鼠尾动脉压及24 h尿蛋白含量;用硫代巴比妥法及黄嘌呤氧化酶法检测大鼠胎盘组织氧化应激指标,流式细胞仪检测大鼠机体细胞免疫功能,蛋白免疫印迹法检测大鼠胎盘组织滋养细胞中X连锁凋亡抑制蛋白(XIAP)表达.结果 孕21 d对照组,模型组和实验组大鼠尾动脉压分别为(119.25±4.21),(136.25±5.48),(123.52±6.45)mmHg;24 h尿蛋白含量分别为(6.42±0.08),(11.83±0.12),(8.58±0.12)mg;胎盘组织中丙二醛(MDA)含量分别为(4.75±0.08),(10.69±0.07),(8.37±0.08)mmol·mg-1,超氧化物歧化酶(SOD)含量分别为(168.97±3.42),(93.26±4.29),(112.63±3.48)U·mg-1;CD4+/CD8+分别为1.46±0.05,2.61±0.56,1.48±0.12,胎盘组织滋养细胞中XIAP蛋白相对表达量分别为0.36±0.09,0.89±0.05,0.41±0.11,模型组分别与对照组、实验组比较,差异均有统计学意义(均P<0.05).结论 葛根素能抑制PE大鼠胎盘氧化应激反应,增强细胞免疫功能,同时其能够降低胎盘组织滋养细胞中XIAP蛋白表达水平,从而抑制胎盘滋养细胞的凋亡.
Objective To investigate the effect of puerarin on oxidative stress and immune status in placenta of preeclampsia (PE) rats. Methods Forty-five pregnant female SD rats were randomly divided into control group,model group and test group,15 rats in each group. The rats in model group and test group were subcutaneously injected with 100 mg·kg-1 of L-arginine methyl ester (L-NAME) once daily from Day 13-21 of gestation,the rats in control group were injected with the same amount of saline; and the rats in test group were intraperitoneally injected with 80 mg·kg-1 of puerarin solution once daily from Day 17-21 of gestation,control group and model group were injected with the same amount of 0. 9% NaCl. The caudal arterial pressure and 24 h urinary protein content were measured at Day 10,16 and 21 of gestation,the oxidative stress indexes in rat placenta was detected by thibabituric acid method and Xanthine oxidase method,the cellular immune function was detected by flow cytometry,and the X-linked inhibitor of apoptosis protein (XIAP) expressionin of rat placenta trophoblasts was detected by Western blot. Results On Day 21 of gestation,there were significant differences between the model group and the control/test group in caudal arterial pressure [(136. 25 ± 5. 48) mm- Hg vs (119. 25 ± 4. 21) mmHg or (123. 52 ± 6. 45) mmHg],24 h-urinary protein contents [((11. 83 ± 0. 12) mg vs 6. 42 ± 0. 08) mg or (8. 58 ± 0. 12) mg],the content of MDA in placenta tissue [(4. 75 ± 0. 08) mmol·mg-1 vs(10. 69 ± 0. 07) mmol·mg-1,(8. 37 ± 0. 08) mmol·mg-1 ],the content of SOD [(93. 26 ± 4. 29) U·mg-1 vs(168. 97 ± 3. 42) U·mg-1 or (112. 63 ± 3. 48) U·mg-1 ],the ratio of CD4 +/CD8 + [(2. 61 ± 0. 56) vs(1. 46 ± 0. 05) or (1. 48 ± 0. 12) ] and the relative expression of XIAP protein in trophoblast [(0. 89 ± 0. 05) vs(0. 36 ± 0. 09) or (0. 41 ± 0. 11) ](all P < 0. 05). Conclusion Puerarin can inhibit the oxidative stress reaction and enhance the cellular immune function in the placenta of PE rats,and reduce the expression of XIAP protein in the placenta tissue trophoblasts to reduce the apoptosis of placenta trophoblasts.