目的:了解潍坊地区尖锐湿疣患者人类乳头瘤病毒( HPV)感染及亚型分布情况,从而为尖锐湿疣的病因诊断、治疗、预后判断和病情监测提供依据。方法采用基因扩增及导流杂交分型技术对潍坊地区460例尖锐湿疣患者疣体脱落细胞进行HPV检测和分型。结果460例就诊患者样本共检出427例HPV阳性,检出率为92.83%,检测出HPV亚型17种,检出率最高的依次为HPV6型占26.46%(113/427),HPV53型占22.01%(94/427),HPV16型占19.67%(84/427),其中单一型感染为52.22%(223/427),204例存在2~4种基因亚型多重感染。结论潍坊地区尖锐湿疣患者HPV感染以HPV6,HPV53,HPV16型感染占多数,单一感染以低危型HPV6为主,多重感染以高低危混合、高危型混合为主。
Objective To realize the Human Papillomavirus( HPV) detection and subtype distribution of Weifang patients with genital warts,which provide a support for the etiological diagnosis,treatment,prognosis and disease monitoring of genital warts.Methods Gene amplification and hybridization typing techniques diversion were used to de-tect HPV and genotype the wart dropping cells of 460 patients with genital warts in Weifang.Results Four hundrend and twenty-seven cases were detected HPV-positive of 460 patients samples,the detection rate was 92.83%(427/460) and 17 HPV species subtypes were detected,in which the highest detection rate in the order of HPV6 type accounted for 26.46%(113/427),HPV53 type accounted for 22.01%(94/427),HPV16 type accounted for 19.67%(84/427),in which single infection was 52.22%(223/427),there were 2~4 subtypes multiple infections in 204 cases.Conclusion Weifang HPV infection of genital warts patients is almostly HPV6,HPV53 and HPV16 subtypes,in which single infectionis priority of low-risk HPV6,multiple infections is priority of high-risk mix and low risk mix.