为研究解整合素-金属蛋白酶9(ADAM9)与藏鸡胚胎低氧适应性的关系,试验对藏鸡和茶花鸡在低氧(O2,13%)和常氧(O2,21%)孵化条件下胚胎尿囊绒毛膜(CAM)组织ADAM9基因mRNA和蛋白表达量进行检测,同时检测了在不同氧气浓度培养条件下鸡胚成纤维细胞(DF-1)中ADAM9基因的mRNA表达变化和ADAM9基因启动子区活性.结果显示:不论藏鸡还是茶花鸡,除第18天外,在低氧孵化下CAM组织中ADAM9的表达量显著高于常氧孵化同一时期(P<0.05),并且在低氧条件下培养的DF-1细胞中ADAM9基因mRNA表达量在36 h时显著高于常氧条件(P<0.05).结果表明低氧能刺激藏鸡胚CAM组织中ADAM9基因的表达,从而使其胚胎能更好地适应低氧环境.
In order to study the relationship between ADAM metallopeptidase domain 9(ADAM9)and the hypoxic adaptabil-ity of Tibetan chicken embryos,the mRNA and protein expression of ADAM9 gene in embryonic chorioallantoic membrane(CAM)tissues of Tibetan chicken and Chahua chicken under hypoxic(O2,13%)and normoxic(O2,21%)incubation conditions were de-tected.The expression of ADAM9 gene in chicken embryonic fibroblast cell(DF-1)cultured in different oxygen concentration was compared and ADAM9 gene promoter activity was identified.The results showed that the expression of ADAM9 in CAM tissues un-der hypoxic incubation was significantly higher than that under normoxic incubation at the same time(P<0.05),except for the 18th day.The expression of ADAM9 mRNA in DF-1 cells cultured under hypoxia condition was significantly higher than that un-der normoxia condition at 36 h(P<0.05).The results indicated that hypoxia could stimulate the expression of ADAM9 gene in CAM tissue of Tibetan chicken embryos,so that the embryos could adapt to the hypoxic environment better.