目的 分析 16 批不同基原的市售鱼鳔中氨基酸及蛋白质成分.方法 建立一种 6-氨基喹啉基-N-羟基琥珀酰亚胺基甲酸酯柱前衍生、高效液相色谱法,测定鱼鳔中 17 种氨基酸含量,并对不同基原鱼鳔中氨基酸类成分进行分析;采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析鱼鳔中的蛋白质分子量分布,并采用蛋白质组学法鉴定鱼鳔中的蛋白质.结果 17 种氨基酸在一定浓度范围内线性关系良好(R2≥0.998 0),平均加样回收率 85.62%~109.60%,精密度、稳定性、重复性RSD均小于 3.5%.16 批鱼鳔氨基酸组成与含量存在差异,氨基酸总含量介于 468.31~620.05 mg/g;从 16 批鱼鳔中共鉴定 688 种蛋白质,包括Ⅰ型在内的胶原蛋白多达 11 种,此外,鱼鳔中还含有较多分子质量52~95 kDa的低丰度蛋白.结论 该研究可为鱼鳔的质量评价和开发利用提供参考.
AIM To study the amino acids and proteins in 16 batches of commercial fish swim-bladders with different origins.METHODS A high performance liquid chromatography method based on pre-column derivatization using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate(AQC)was developed for the determination of contents and components of 17 amino acids in fish swim-bladders.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was performed to analyze the molecular weight distribution of proteins from different fish swim-bladders,and proteins in fish swim-bladders were identified by proteomics method.RESULTS The result showed that the determination of 17 amino acids had a good linear relationship(R2≥0.998 0).The average recovery rate was 85.62%-109.60%and the relative standard deviations of precision,stability and repeatability were less than 3.5%.The total content of the 17 amino acids in 16 batches of fish swim-bladders ranged from 468.31 mg/g to 620.05 mg/g.A total of 688 proteins including 11 collagens were identified from 16 batches of fish swim-bladder samples and a plenty of low-abundance proteins at 52-95 kDa were also detected in fish swim-bladders by SDS-PAGE.CONCLUSION This study provides a good reference for the quality evaluation and further utilization of fish swim-bladders.