以绿色廉价的黑米为碳源,采用简单的水热法一步合成了碳点(carbon dots,CDs).所合成的CDs不仅对pH、NaCl溶液和温度变化不敏感,并且不受多种金属离子的影响,表现出优异的稳定性.同时,CDs在较短波长范围(410~470 nm)表现出激发不依赖的发射行为,在较长波长范围(470~510 nm)又表现出弱激发依赖的发射行为.由于其优异的荧光特性、稳定性和低毒性,我们将其应用于人口腔角质形成细胞(Hok)、宫颈癌细胞(Hela)和骨髓间充质干细胞(MC3T3)的体外成像.
Carbon dots(CDs)were synthesized by a facile hydrothermal method using cheap black rice as a carbon source.The synthesized CDs exhibited excellent stability,which was not sensitive to changes in temperature,pH,and solution of NaCl,and were immune to many metal ions.The purified CDs also had low cytotoxicity against human oral keratinocytes(Hok),cancer cells(cervical cancer,Hela),and bone marrow mesenchymal stem(BMSC,MC3T3)cells.Meanwhile,it behaved with excitation-independent behavior in the shorter wavelength range(410-470 nm)and excitation-dependent behavior in the longer wavelength range(470-510 nm).Due to the excellent fluo-rescence properties and stability,we applied them to the intracellular imaging of Hok,Hela,and MC3T3 cells.