异戊烯基转移酶(isopentenyltransferase,IPT)是细胞分裂素生物合成的第一限速酶,为分析IPT基因家族在白菜型油菜生长中的功能,利用生物信息学方法从其基因组中鉴定出13个BrIPT基因,它们不均匀地分布在7条染色体上.BrIPTs可分为4个亚族,各家族成员均含有8~10个保守基序和1~2个UTR区.BrIPT基因的启动子区域包含众多响应元件.BrIPT基因家族成员受环境因子、生物激素及防御与应激调控.qRT-PCR结果显示,tRNA-IPT基因BrIPT4、BrIPT6、BrIPT9在白菜型油菜体内各个部位均有表达.相比于苗期,成熟期白菜型油菜IPT基因的表达量更高,为后续深入研究IPT基因家族成员的生理生化功能提供了理论依据.
Isopentenyltransferase(IPT)is the first limiting enzyme for cytokinin biosynthesis,so to analyze the function of the IPT gene family in Brassica rapa L.,a total of 13 BrIPT genes were identified from the genome of Brassica rapa L.by bioinformatics,which were heterogeneously distributed on 7 chromosomes.These BrIPT genes were divided into 4 substrates and each BrIPT gene included 8~10 conserved motifs and 1~2 UTR regions.The promoter regions of BrIPT genes contained numerous responsive elements.The BrIPT genes were regulated by environmental factors,biohormones,defense and stress.The qRT-PCR results showed that the tRNA-IPT genes BrIPT4,BrIPT6 and BrIPT9 were expressed in various parts of Brassica rapa L..The expressions of IPT genes in Brassica rapa L.were higher at the mature stage than those at the seedling stage.The above results provided some basis for subsequent in-depth studies on the physiological and biochemical functions of IPT gene family members.