目的:建立三妹木药材的质量标准.方法:通过对广西境内10批次不同产地三妹木切片和粉末进行观察,构建其植物学特征;采用薄层色谱法对药材进行定性鉴别,并从薄层色谱法的提取溶剂、提取方法、展开系统等进行考察;参照2020年版《中华人民共和国药典》(四部)通则方法,对水分、灰分等项目进行研究.结果:明确三妹木药材的性状及显微特征;建立了三妹木中黄酮类成分牡荆素、异牡荆素的薄层色谱鉴别方法;综合比较分析三妹木各批药材的检测数据拟定检测限度,定水分、总灰分和酸不溶性灰分依次不得过10.0%、6.0%和1.0%.结论:通过对三妹木药材比较系统的研究,可为三妹木质量标准的建立提供依据,为三妹木进一步开发利用奠定基础.
Objective:To establish the quality standard of Lespedeza Formosa.Methods:The botanical characteristics of Lespedeza Formosa from 10 batches from different origins in Guangxi were constructed by observing the character and microscopic;TLC was used to qualitatively identify the medicinal materials,and the extraction solvent,extraction meth-od and development system of TLC were investigated;According to the general principles and methods of Chinese Pharmacopoeia(Volume Ⅳ)(2020 Edition),the water content,ash content and other items were studied.Results:The characters and microscopic characteristics of Lespedeza Formosa were identified;A TLC method for the identifica-tion of vitexin and isovitexin,the flavonoid components in Lespedeza Formosa,has been established;According to the comprehensive comparison and analysis of the test data of each batch of Lespedeza Formosa,the detection limit was proposed,and the tentative moisture content shall not exceed 10.0%,the total ash content shall not exceed 6.0%,and the acid-insoluble ash content shall not exceed 1.0%.Conclusion:The systematic study on the medicinal materials of Lespedeza Formosa can provide the basis for the establishment of the quality standard of Lespedeza Formosa and lay the foundation for the further development and utilization of Lespedeza Formosa.