丹七片干预心肌缺血的血浆蛋白质组学研究 / Effect of Danqi Tableton Plasma Proteome in Swines with Myocardial Ischemia
- Resource Type
- Academic Journal
- Authors
- 郭淑贞; 啜文静; 王勇; 冯玄超; 廉洪建; 郑磊; 王伟
- Source
- 中西医结合心脑血管病杂志 / Chinese Journal of Integrative Medicine on Cardio/Cerebrovascular Disease. (11):1355-1357
- Subject
- 心肌缺血
丹七片
血浆蛋白质组学
Danqi table t
myocardial ischem ia
plasma proteome
- Language
- Chinese
- ISSN
- 1672-1349
目的:探讨丹七片干预心肌缺血的作用机制。方法缩窄环法制备小型猪心肌缺血模型,给予丹七片每次9 g /(只?天)临床等效剂量,4周。双向凝胶电泳质谱联用法分析血浆蛋白表达谱变化。结果与模型组比较,丹七片下调蛋白点9个(丰度比值≤0.5)、上调蛋白点11个(比值≥1.5)。鉴定出的上调蛋白为载脂蛋白 A1和载脂蛋白 E。结论通过上调载脂蛋白 A1和载脂蛋白 E抑制缺血心肌过度的炎症反应,可能是丹七片干预心肌缺血的主要机制之一。
Objective To explore the effect of Danqi table t(DQT) on plasma proteome in sw ines w ith myocardial ischem ia. Me thods Myocardial ischem ia was induced by a constrictoron the left coronary artery.After treated by DQT w ith a dose of 9 g per day for4 weeks ,two dimension e lectrophoresis andmass spectrome terwas used to analyze the changes ofplasma proteome .Re-sults The abundances of 9 prote in spots were decreased while 11 prote in spots increased in animals treated by DQT.Apolipopro-te in A1 and apolipoprote in E were identified as increased prote ins .Conclusion DQTmay negative ly regulate the excess inflamma-tion induced by myocardial ischem ia and it is proved by increase plasma apolipoprote in A1 and apoliopoprote in E.