目的 观察Ⅰ组代谢型谷氨酸受体(mGluR1/5)在大鼠颈上神经节(SCG)组织的表达、定位,及慢性间歇性低氧(CIH)对 mGluR1/5 蛋白水平的影响.方法 12 只雄性 SD 大鼠随机分为对照组(Ctrl)和 CIH 组(CIH),每组 6 只,模型制作 6 周.Western blotting检测CIH对mGluR1/5 蛋白水平的影响,免疫组织化学法和免疫荧光共定位染色检测大鼠SCG中mGluR1/5 的表达及分布.结果 mGluR1/5 表达于大鼠SCG,mGluR1 分布于神经元和小强荧光(SIF)细胞,而在卫星胶质细胞(SGCs)、神经纤维和血管不表达;mGluR5 主要分布于神经纤维,少量分布于神经元,而在SIF细胞、SGCs及血管不表达;CIH上调mGluR1/5 的蛋白水平(P<0.01).结论 mGluR1和mGluR5 均表达于大鼠SCG,但其表达部位有所不同,两者蛋白水平的升高可能参与了CIH诱导的血压升高.
Objective To observe the expression and localization of group Ⅰ metabotropic glutamate receptors(mGluR1/5)in rat superior cervical ganglion(SCG)and the effect of chronic intermittent hypoxia(CIH)on mGluR1/5 protein level.Methods Twelve male SD rats were randomly divided into control group(Ctrl)and CIH group(CIH),6 rats in each group.After 6 weeks of modeling,the effect of CIH on mGluR1/5 protein level was detected by Western blotting,the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining.Results mGluR1/5 was expressed in rat SCG.mGluR1 was distributed in neurons and small intensely fluorescent(SIF)cells,but not in satellite glial cells(SGCs),nerve fibers and blood vessels,whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons,but not in SGCs,SIF cells and blood vessels.CIH increased the protein levels of mGluR1/5(P<0.01)in rat SCG.Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG,but their distribution are different,and the increased protein levels of both may be involved in CIH-induced hypertension.