傣药"雅解"是傣族传统医学中最具特色的药物和治疗方法.其中,宾蒿(Clerodendrum chinense var.sim-plex)、傣百解(Marsdenia tenacissima)和竹叶兰(Arundina graminifolia)作为3种常见的"雅解"被广泛使用.本研究使用石油醚、乙酸乙酯、正丁醇或水依次萃取3种"雅解"的乙醇提取物获得各极性部位,并制备3种"雅解"的水煎剂.经测定3种"雅解"的总黄酮质量分数为22.41~586.39 mg/g,高于总多酚质量分数(2.76~28.66 mg/g).3种"雅解"的提取物均可清除 DPPH、·OH 和 ABTS自由基,其中对ABTS自由基的清除率高于其他自由基(IC50>380 μg/mL).它们对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的抑制作用较弱.此外,3种"雅解"具有一定的抗炎作用,在60 μg/mL 的质量浓度下即可显著下调脂多糖刺激巨噬细胞产生的NO、肿瘤坏死因子-α、白细胞介素-1β 和白细胞介素-6.综上所述,本研究通过现代科学手段初步揭示了3种"雅解"的解毒作用机理,为"雅解"的进一步优化与研究奠定了基础.
Dai antidotes are the most distinctive medicine and treatment in traditional Dai medicine.Bin Hao(Clerodendrum chinense var.simplex),Dai Bai Jie(Marsdenia tenacissima)and Zhu Ye Lan(Arun-dina graminifolia)are three Dai antidotes widely used for their"detoxifying effects",and their use is rooted in a theoretical system significantly different and much less understood than Western or tradi-tional Chinese medicines.Here,we successively extracted the three Dai antidotes using petroleum ether,ethyl acetate,n-butanol,or water,and then prepared their decoctions.The content of total flavo-noids in three Dai antidotes ranged from 22.41 to 586.39 mg/g,which is higher than the content of total polyphenols(2.76 to 28.66 mg/g).The various extracts were found to scavenge radicals of DPPH,·OH and ABTS.They scavenged ABTS radicals much more efficiently than other radicals(IC50>380 μg/mL).They weakly inhibited the growth of E.coli,P.aeruginosa and S.aureus.Notably,even at low concentra-tion 60 μg/mL,the extracts can significantly down-regulate the production of NO,TNF-α,IL-1β,and IL-6 by macrophages stimulated with LPS.In conclusion,our results provide the first mechanistic insights into the detoxifying effects of three Dai antidotes,providing a foundation for their optimization and for future research to strengthen Dai medicine through modern scientific practices.