[目的]了解 Oleosin基因功能,初步探索Oleosin基因家族与谷子耐旱性的关系,挖掘谷子抗旱基因.[方法]以抗旱品种勾勾母鸡咀(GG)和干旱敏感品种晋汾16(JF16)为试验材料,在苗期进行 PEG模拟干旱胁迫处理后,对其表达谱进行分析.进而对谷子Oleosin基因家族进行生物信息学分析.[结果]在干旱胁迫下,Oleosin基因Seita. 9G579700在GG和JF16两个品种中均显著上调表达;谷子 Oleosin基因家族较小,只有6个成员,分子量在15~18 kDa;系统发育树分析结果为,谷子Oleosin基因家族与单子叶植物玉米、高粱、水稻聚为一类,而与双子叶拟南芥则亲缘关系较远;启动子元件分析表明,谷子6个Oleosin基因均含有光响应、ABA响应、MeJA响应以及MYB结合位点等与抗旱相关的顺式作用元件.[结论]初步确定Oleosin基因是调控谷子干旱胁迫的候选基因,可能受干旱或ABA、MeJA诱导表达,这为进一步深入研究 Oleosin基因功能及其与谷子耐旱性的关系提供理论参考.
[Objective]To understand the relationship between Oleosin gene family and drought tolerance in foxtail millet,and explore gene related to drought tolerance.[Methods]In this study,drought-tolerant variety Gou Gou Mu Ji Ju(GG)and drought-sensitive variety Jinfen 16(JF16)were used as experimental materials.They were treated by PEG at seedling for simulating drought stress and expression analysis was performed.In order to understand the Oleosin gene family in foxtail millet,bioinformatics analysis was conducted.[Results]The result showed that the Oleosin gene(Seita.9G579700)was sig-nificantly up-regulated in both GG and JF16 under drought stress.The Oleosin gene family was small and had only 6 mem-bers with molecular weight of 15~18 kDa.Phylogenetic tree analysis showed that Oleosin family in foxtail millet were clus-tered with monocotyledonous,such as maize,sorghum and rice,but the relationship with dicotyledonous A rabidopsis thali-ana was significant distant.The analysis of promoter elements showed that the six Oleosin genes in foxtail millet contained light response,ABA response,MeJA response,and MYB binding sites which were associated with drought tolerance.[Con-clusion]It could be proposed that the Oleosin gene was a candidate gene for regulating drought stress response in foxtail mil-let.Oleosin gene may be induced by drought or ABA,and MeJA.This study could provide a theoretical reference for further research on the understanding the function of Oleosin genes and its relationship with drought tolerance in foxtail millet.