[目的]金属纳米材料的一经问世,便展现出优异的高效性,新型金属纳米抑菌材料在农业领域的应用仍需探索.[方法]以CuSO4 与FeCl3 为原料,研究Cu-Fe双金属纳米粒子(Cu-Fe NPs)的制备工艺,采用菌丝生长速率法测定了Cu-Fe NPs对几种常见植物病原菌的抑菌活性.[结果]将 10 mLCuSO4 与FeCl3 的混合液以 4 mL/min的速度滴加到30 mL pH为 11 浓度为0.001 mol/L的儿茶酚溶液中,超声反应30 min,可以获得形貌不规则,粒径分布均一,平均粒径 30 nm的Cu-Fe NPs.Cu-Fe NPs能显著抑制几种常见的植物病原菌的生长,对黄瓜疫病菌(Phytophthora melonis)、番茄早疫病菌(Alternaria solani)、苹果腐烂病菌(Valsa mali)和萝卜黑斑病菌(Alternaria aphanin)的EC50分别为325.05、334.00、429.64 和325.62 mg/L.[结论]Cu-Fe NPs具有良好的抑菌潜力,研究结果可为金属纳米粒子防治植物病原病害提供数据支撑,并为纳米农药的应用提供参考.
[Objective]The emergence of metal nanomaterials has demonstrated excellent efficiency,and the application of new metal nanomaterials for antibacterial purposes in agriculture still needs to be explored.[Method]In this study,the chemical reduction method was used to prepare Cu-Fe NPs with excellent antifungal activities.The mycelial growth rate method was employed to study the Cu-Fe NPs against several common plant pathogenic microorganisms.[Result]The study showed that the mixed solution of 10 mL CuSO4 and FeCl3 was dropped into 30 mL catechol solution(0.001 mol/L)with pH of 11 at a rate of 4 mL/min,and the ultrasonic reaction lasted for 30 min,which could obtain Cu-Fe NPs with irregular morphology,uniform particle size distribution and an average particle size of 30 nm.Cu-Fe NPs could significantly inhibit the mycelial growth of Phytophthora melonis,Alternaria solani,Valsa mali and Alternaria aphanin with EC50 values of 325.05,334.00,429.64 and 325.62 mg/L,respectively.[Conclution]Cu-Fe NPs have good potential to inhibit the growth of pathogenic microorganisms,and the research results can provide data support for the prevention and treatment of fungal diseases with metal nanoparticles,and provide references for the application of nano-pesticides.