Absence of Helicobacter pylori in Pediatric Adenoid Hyperplasia
- Resource Type
- Authors
- Damian J. Hussey; Penelope K. Harris; A. Simon Carney; Charmaine M. Woods; Eng H. Ooi; Anthony Thomas
- Source
- Archives of Otolaryngology–Head & Neck Surgery. 137:998
- Subject
- Pathology
medicine.medical_specialty
biology
medicine.diagnostic_test
business.industry
General Medicine
Hyperplasia
Helicobacter pylori
Adenoid
medicine.disease
biology.organism_classification
Reverse transcriptase
law.invention
Laryngopharyngeal reflux
medicine.anatomical_structure
Otorhinolaryngology
law
Biopsy
medicine
Surgery
Primer (molecular biology)
business
Polymerase chain reaction
- Language
- ISSN
- 0886-4470
Objectives To (1) develop a reverse transcription-polymerase chain reaction assay to determine whether Helicobacter pylori and/or other members of the Helicobacteraceae family are detected in hyperplastic adenoids of children and (2) critically analyze published polymerase chain reaction methods to ascertain whether false-positive detection of H pylori has been reported. Design Cohort study. Patients Adenoid biopsy specimens (78 hyperplastic and 15 normal) were collected from children aged 2 to 10 years. Methods Total RNA was extracted before reverse transcription of bacterial RNA using Helicobacteraceae-specific primer. A nested reverse transcription–polymerase chain reaction protocol was designed to detect all species of the Helicobacteraceae family. A piece of each biopsy specimen was examined histologically. Results Laryngopharyngeal reflux was suspected in 41% of the children (n = 23) on the basis of the Reflux Symptom Index. No evidence of H pylori was found in any adenoid sample. Candidatus Wolinella africanus was the only Helicobacteraceae family member detected in 1 hyperplastic adenoid. Histologic examination identified very few bacterial organisms. Previous polymerase chain reaction findings may be the result of false-positive H pylori detection. Conclusions Inflammation and enlargement of the adenoids is not likely due to ongoing bacterial infection arising from laryngopharyngeal reflux. We conclude that H pylori and other Helicobacteraceae family members are not major contributors to the development of hyperplastic adenoids in children.