Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes
- Resource Type
- Authors
- Maria Carla Gilardi; Alessandra Alberti; Antonino La Magna; Ruggero Anzalone; Giuseppe Fisicaro; Valentina Bravatà; Andrea Ballo; Francesco La Via; Giorgio Ivan Russo; Luigi Minafra; Gianluca Giustolisi; Giusi Irma Forte; Massimo Camarda; Silvia Scalese; Francesco Paolo Cammarata
- Source
- BioMedical Engineering
BioMedical engineering online (Online) 13 (2014): 71. doi:10.1186/1475-925X-13-71
info:cnr-pdr/source/autori:Massimo Camarda 1; Giuseppe Fisicaro,; Ruggero Anzalone 1; Silvia Scalese 1; Alessandra Alberti 1; Francesco La Via 1; Antonino La Magna 1; Andrea Ballo 2; Gianluca Giustolisi 2; Luigi Minafra 3; Francesco P. Cammarata 3; Valentina Bravatà 3; Giusi I. Forte 3; Giorgio Russo 3; Maria Carla Gilardi/titolo:Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes/doi:10.1186%2F1475-925X-13-71/rivista:BioMedical engineering online (Online)/anno:2014/pagina_da:71/pagina_a:/intervallo_pagine:71/volume:13
- Subject
- Electrophoresis
Polynomial
Monte Carlo method
Biomedical Engineering
Cell Communication
cell-cell dipole
Molecular physics
Quantitative Biology::Cell Behavior
Biomaterials
Polarizability
Cell Line, Tumor
Electric field
Electric Impedance
Electronic engineering
Humans
Radiology, Nuclear Medicine and imaging
Poisson Distribution
Electrodes
Physics
Radiological and Ultrasound Technology
Research
General Medicine
Dipole
Distribution (mathematics)
Electrode
Monte Carlo Method
Algorithms
- Language
- English
- ISSN
- 1475-925X
BACKGROUND: We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices. METHODS: We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions. RESULTS: The real and simulated distributions are consistent. In both cases the cells distribution near the electrodes is dominated by cell-cell dipole interactions which generate long chains. CONCLUSIONS: The agreement between real and simulated cells' distributions demonstrate the method's reliability. The distribution are dominated by cell-cell dipole interactions even at low density regimes (105 cell/ml). An improved estimate for the density threshold governing the interaction free regime is suggested.