Additional file 4: Figure S4. Silence of Atg5 or Atg7 did not affect the anticancer activity of BKM120 and other PI3K inhibitors. (A) Western blot analysis of Atg5 and Atg7 in SKOV-3 and MKN-1 cells after transfection with non-targeting siRNA (siNT), Atg5 and Atg7 siRNA, respectively. (B) Cell viability of SKOV-3 and MKN-1 cells after transfected with Atg7 siRNA, followed by treatment of BKM120 for 72 h. (C) Colony forming ability of SKOV-3 and MKN-1 cells after transfected with non-target siRNA or Atg7 siRNA and treated with BKM120 for 72 h and allowed to recover in fresh media for 7���10 days. Graph of colony quantification is shown in (D). (E) Cell viability of MKN-1 cells after transfected with Atg5 or Atg7 siRNA, followed by treatment of BAY 80-6946 or GDC-0941 for 72 h. Graphs are normalized to 100% per treatment and shown as mean �� SD from three independent experiments; ns: p > 0.05.