Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation
- Resource Type
- Authors
- James McGinty; Joseph V. Hajnal; Vadim Y. Soloviev; Daniel W. Stuckey; Alessandro Sardini; Dominic J. Wells; Marzena Wylezinska-Arridge; Simon R. Arridge; Paul M. W. French; Romain F. Laine
- Source
- Scopus-Elsevier
- Subject
- Optics and Photonics
Photon
Materials Science (miscellaneous)
0205 Optical Physics
HIGHLY SCATTERING INCLUSIONS
Article
Fluorescence
Industrial and Manufacturing Engineering
Mice
Imaging, Three-Dimensional
Optics
FLUORESCENCE LIFETIME TOMOGRAPHY
Fluorescence Resonance Energy Transfer
Radiative transfer
Animals
Scattering, Radiation
Tomography, Optical
Business and International Management
Absorption (electromagnetic radiation)
Photon diffusion
Physics
Science & Technology
Scattering
business.industry
0906 Electrical And Electronic Engineering
Models, Theoretical
Ray
Diffuse optical imaging
Förster resonance energy transfer
TURBID MEDIA
Physical Sciences
OPTICAL TOMOGRAPHY
TRANSFER PARAMETERS
business
Algorithms
0913 Mechanical Engineering
- Language
- ISSN
- 1539-4522
0003-6935
We describe a new light transport model that we have applied to 3-D image reconstruction of in vivo fluorescence lifetime tomography data applied to read out Förster Resonance Energy Transfer in mice. The model is an approximation to the Radiative Transfer Equation and combines light diffusion and rays optics. This approximation is well adopted to wide-field time-gated intensity based data acquisition. Reconstructed image data are presented and compared with results obtained by using the Telegraph Equation approximation. The new approach provides improved recovery of absorption and scattering parameters while returning similar values for the fluorescence parameters.