Additional file 3: Figure S1. The distribution of 17 included screens which focused on investigating the regulators of immune cell-mediated killing among different screening types, library types, organisms, and algorithms. Figure S2. Results of Functional similarity analysis. Figure S3. Distribution of up- (red) and down- (blue) regulated genes (between tumor tissues and adjacent tissues) of sensitizers and resistors across different TCGA cancer types. Figure S4. The ratio between the inactivation event numbers of sensitizers and resistors. Figure S5. Functional characterization of sensitizers and resistors in each cancer type. Figure S6. Experimental validation of the function of MON2. Figure S7. The overall characterization of MON2. Figure S8. Relationship between MON2 and microsatellite instability (MSI). Figure S9. Comparison of the performance for predicting response to immunotherapy between CTIS and 14 published signatures in pretreatment datasets. Figure S10. Characterization of CTIS in TCGA Pan-Cancer cohort. Figure S11. Summary of ICB-treated CRISPR screens.