Additional file 1: Figure S1. (A) Liver-to-body ratio, serum ALT, AST, T-CHO, and TG levels in liver tissue from EtOH-fed mice. (B, C) Representative images of H&E staining in liver tissue from EtOH-fed and PT2399-treated mice (scale bar = 100 μm or 20 μm) (n = 6). (D) Western blotting analysis of Hif-2α expression in the nucleus and cytoplasm in EtOH-treated AML-12 cells. Representative images of Hif-2α (green) and nuclei (blue) in EtOH-treated AML-12 cells (scale bar = 15 μm) (n = 3). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01. Figure S2. (A) Triglyceride (TG) levels in EtOH-treated AML-12 cells. (B) MTT assay, the level of TGs, and qRT-PCR analysis of VEGF expression in AML-12 cells at different concentrations of PT2399 (n = 3). (C) Liver-to-body ratio, serum T-CHO, ALT, and AST levels in the liver tissue of PT2399-treated mice (n = 6). (D, E) Western blotting analysis of BNIP3, Beclin1, and LC3II/LC3I expression in EtOH-treated AML-12 cells, and Hif-2α-siRNA and BNIP3-shRNA co-treated AML-12 cells (n = 3). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01. Figure S3. (A) Representative images of mitochondria (red), LC3 (green), and nuclei (blue) in EtOH-treated AML-12 cells. (B, C) Representative images of BNIP3 (red), LC3 (green), and nuclei (blue) in EtOH-treated and PT2399-treated AML-12 cells (scale bar = 15 μm) (n = 3). (D) Representative TEM images of lipid droplets (LDs), mitochondria, and autophagosomes in the liver tissue of EtOH-fed mice. Black arrows denote autophagosomes, yellow arrows denote LDs, and red arrows denote mitochondria (n = 6). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01. Figure S4. (A) Western blotting analysis of BNIP3, Beclin1, and LC3II/LC3I expression in pc-DNA3.1-BNIP3-treated AML-12 cells. (B) Western blotting analysis of CPT-1α and MCAD expression in Hif-2a-siRNA and BNIP3-shRNA co-treated AML-12 cells (n = 3). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01. (C) Representative images of mitochondria (red), LC3 (green), and nuclei (blue) in pc-DNA3.1-BNIP3-treated AML-12 cells. (D) Representative images of BNIP3 (red), LC3 (green), and nuclei (blue) in pc-DNA3.1-BNIP3-treated AML-12 cells (scale bar = 15 μm).