Development of a Pre‐assembled Through‐Bond Energy Transfer (TBET) Fluorescent Probe for Ratiometric Sensing of Anticancer Platinum(ll) Complexes
- Resource Type
- Authors
- Wee Han Ang; Jun Xiang Ong
- Source
- Chemistry – An Asian Journal. 15:1449-1455
- Subject
- Organoplatinum Compounds
Energy transfer
chemistry.chemical_element
Antineoplastic Agents
010402 general chemistry
01 natural sciences
Biochemistry
Fluorescence microscope
Humans
Bond energy
Fluorescent Dyes
Molecular Structure
010405 organic chemistry
Chemistry
Optical Imaging
Organic Chemistry
General Chemistry
Prodrug
Combinatorial chemistry
Fluorescence
Ratiometric fluorescence
0104 chemical sciences
Energy Transfer
Microscopy, Fluorescence
Cisplatin
Platinum
HeLa Cells
- Language
- ISSN
- 1861-471X
1861-4728
Fluorescence microscopy has emerged as an attractive technique to probe the intracellular processing of Pt-based anticancer compounds. Herein, we reported the first through-bond energy transfer (TBET) fluorescent probe NPR1 designed for sensitive detection and quantitation of PtII complexes. The novel TBET probe was successfully applied for ratiometric fluorescence imaging of anticancer PtII complexes such as cisplatin and JM118 in cells. Capitalizing on the ability of the probe to discriminate between PtII complexes and their PtIV derivatives, the probe was further applied to study the activation of PtIV prodrug complexes that are known to release active PtII species after intracellular reduction.