Additional file 8: Figure S5. Characterization of cesa3-3. (A) Representative images of 7 day-old WT, cesa3-3 and cev1 seedlings. (B) Primary root length box plots of indicated genotypes. Medians are represented inside the boxes by solid lines, and circles depict individual measurements (n = 38-61). (C) Representative primary root images specifying the initiation of the differentiation zone by the appearance of root hairs (dashed line), and bulging cells in cesa3-3 and cev1 (asterisks). (D) Box plot summary of primary root diameter at the onset of differentiation (n = 31). (E) Lignin deposition visualized by phloroglucinol stain (fuchsia) in primary roots of indicated genotypes. Letters in (B, D) denote statistically significant differences among samples determined by ANOVA followed by Tukey’s HSD test. Scale bar:100 μm (E).