Background Noninvasive laboratory tests have been widely used in the diagnosis of liver fibrosis. This study was designed to evaluate the diagnostic value of the four serum markers detected on a JETLIA-962 chemiluminescence analyzer and the FibroTest index for chronic hepatitis B (CHB) related liver fibrosis. J. Clin. Lab. Anal. 27:5–11, 2013. © 2012 Wiley Periodicals, Inc. Methods A JETLIA-962 chemiluminescence analyzer was used to measure the hyaluronic acid (HA), laminin (LN), procollagen III N-terminal peptide (PIIINP), and type IV collagen (CIV) contents in the sera of CHB patients and controls. Results In our research, it was found that serum HA, LN, PIIINP, and CIV concentrations and the FibroTest index were correlated with fibrosis stage. The FibroTest index had the largest area under the receiver operating characteristic (ROC) curve for fibrosis and cirrhosis (0.80 and 0.776, respectively). The five indices together were able to exclude fibrosis with a negative likelihood ratio