Figure S1. Simplified scheme of the examined bioenergetic pathways in the studied IDH1 wild-type and mutant glioma cells. In our study, different energy substrate oxidation and metabolic enzymes relating to the following bioenergetic pathways were analysed regarding the role of IDH1 mutation. Energy substrate oxidation: glucose, glutamine, citrate, GABA, acetate, malate, lactate and glutamine were measured by Seahorse technique. Energy metabolites - succinate, fumarate, malate, citrate, α-ketoglutarate, glutamate and 2-hydroxyglutarate were determined by liquid chromatography-mass spectrometry. Several protein expressions were measured by Western blot analysis or immunohistochemistry - Glycolysis: hexokinase 2 (HK2), phosphofructokinase P (PFKP), Glutaminolysis: alanine, serine, cysteine-preferring transporter 2 (ASCT2), glutaminase (Gls), GABA shunt: GABA transporter (GAT1), succinic semialdehyde dehydrogenase (SSADH), acetate consumption: acetyl-CoA synthetase 2 (ACSS2). Other abbreviations can be found in the figure: GLUT1: glucose transporter 1, IDH: isocitrate dehydrogenase, LDH: lactate dehydrogenase, MCT1: monocarboxylate transporter 1, OAC: oxaloacetate, SSA: succinic semialdehyde. (PDF 348 kb)