Background Female carriers of dystrophin gene mutations (DMD-FC) were previously considered non-manifesting, but in recent decades, cardiomyopathy associated with muscular dystrophy and myocardial fibrosis has been described. Our study aimed to assess prospectively myocardial fibrosis in asymptomatic DMD-FC compared to a sex- and age-matched control group (CG) using T1 native mapping and extracellular volume (ECV) by cardiovascular magnetic resonance (CMR) imaging. To our knowledge, this approach has not been published yet. Material and methods 38 DMD-FC (mean age 39.1 ± 8.8 years) and 22 CG (mean age 39.9 ± 12.6 years) were included. Using CMR, T1 native relaxation time and ECV were determined in each group. Late gadolinium enhancement (LGE) was assessed in all cases. Results T1 native relaxation time was similar for DMD-FC and CG (1005.1 ± 26.3 ms vs 1003.5 ± 25.0 ms; p-value = 0.81). Likewise, the global ECV was also similar between the groups (27.92 ± 2.02% vs 27.10 ± 2.89%; p-value = 0.20). The segmental analysis of ECV according to the American Heart Association classification did not show any differences between DMD-FC and CG. Conclusion There were no statistically significant differences in the global T1 native relaxation time and global or segmental ECV.