Additional file 3: Figure S3. Neuronal cell death is not prominent in heads of 25-day-old Adar5G1 mutant flies. (A) TUNEL staining to detect apoptotic cells in head sections from 25-day-old Adar5G1 mutant flies stained with DAPI to detect nuclei. TUNEL-positive nuclei are not detected in neurons However TUNEL-positive nuclei are conspicuous in head fat bodies of 25-day-old Adar5G1 mutant flies (boxed area in A). (B) Magnification of area boxed in A (C) Haematoxylin and eosin stained section serial to A, white box indicates fat body tissue. (D) Magnification of area boxed in C. (E, F) Images show representative 6 micron thick haematoxylin and eosin stained sections through mushroom body calyces (left panels, (63X)) and retinas (right panels, 40X) of 30-day Adar5G1; ChAT>UAS-p35. Scale bars: 20 μm.