Chromatography Paper Strip Method for Collection, Transportation, and Storage of Rotavirus RNA in Stool Samples
- Resource Type
- Authors
- Piet Maes; Marc Van Ranst; Goutam Podder; Jelle Matthijnssens; Karolien De Leener; Truus Goegebuer; Mustafizur Rahman; Tasnim Azim
- Source
- Journal of Clinical Microbiology. 42:1605-1608
- Subject
- Diarrhea
Rotavirus
Microbiology (medical)
Rotavirus RNA
Chromatography, Paper
Biology
medicine.disease_cause
Rotavirus Infections
Specimen Handling
Feces
fluids and secretions
Virology
medicine
Humans
Diagnostic laboratory
Edetic Acid
Reagent Strips
Reagent strip
Chromatography
Filter paper
Reverse Transcriptase Polymerase Chain Reaction
Sodium Dodecyl Sulfate
Paper chromatography
Epidemiological surveillance
RNA, Viral
Sample collection
- Language
- ISSN
- 1098-660X
0095-1137
We developed a novel method that uses sodium dodecyl sulfate-EDTA-treated chromatography paper strips to collect unconcentrated fresh stool samples. After the paper strips were stored for 4 months at room temperature, rotavirus RNA could be successfully amplified by using reverse transcriptase PCR. The use of filter paper strips as a specimen support allows (self-)collection of stool samples by untrained persons. Diarrheal stool samples from remote areas can be stored and transported to a central diagnostic laboratory without the need for freezers or special shipping conditions. This convenient and inexpensive rotavirus sample collection system can be of use in epidemiological surveillance studies and vaccine trials.