Additional file 9: Figure S7. In silico analysis of Cas9-mediated INDELs in HTR5A. (A) The frequency of in-frame (mutations) and frameshift mutations among INDEL-containing reads in the genomic DNA of dox-induced clones. (B) The upper and lower limits of amino acid insertions/deletions in mutant reads. (C) Examples of INDEL translational consequences. Red arrows indicate corresponding Cas9 cleavage site in genomic DNA. The red X indicates a deleted amino acid whereas amino acids insertions are coloured blue. The * indicates a nonsense mutation.