Identification and heterologous expression of an isoquinolinequinone biosynthetic gene cluster from Streptomyces albus J1074
- Resource Type
- Authors
- Zhiyang Feng; Shumao Chai; Mingming Cao
- Source
- Biochemical and biophysical research communications. 540
- Subject
- 0301 basic medicine
Biophysics
Biochemistry
Genome
03 medical and health sciences
0302 clinical medicine
Gene cluster
Molecular Biology
Streptomyces albus
Soil Microbiology
Biological Products
biology
Chemistry
Quinones
Cell Biology
Gene Expression Regulation, Bacterial
biology.organism_classification
Isoquinolines
Small molecule
Streptomyces
Biosynthetic Pathways
030104 developmental biology
Genes, Bacterial
030220 oncology & carcinogenesis
Multigene Family
Genome mining
Identification (biology)
Heterologous expression
Function (biology)
- Language
- ISSN
- 1090-2104
Nitrogen heterocycle small molecules display various pharmaceutically important bioactivities and have great potential in drug development and application. Microbes are an important source for discovering nitrogen heterocycle natural products, and the elucidation of their biosynthetic pathways in microbes facilitates genetic manipulation of new nitrogen heterocycle products. In this study, we isolated three isoquinolinequinones from a Streptomyces albus J1074 conjugant and identified their biosynthetic gene cluster in the S. albus J1074 genome. The function of the biosynthetic gene cluster was confirmed by heterologous expression of the gene cluster in S. coelicolor M1146. This study uncovered a new biosynthetic machinery to produce nitrogen heterocycle natural products in microbes.