Impact of nucleotide difference threshold on the performance of the cleaning procedure. (A-D) The barcodes left after cleaning when using variable nucleotide difference thresholds are compared to the barcodes that are true according to the reference list of the viral barcode library. Considering the reference list as a gold standard, the sensitivity, specificity and precision are shown for each of the four individual lanes. Note that sensitivity declines with increasing threshold, whereas specificity and precision increase. (PDF 29 kb)