Towards lab-on-card, label-free enzyme immunoassays based on electrical impedance spectroscopy
- Resource Type
- Authors
- VREYS, Frederik; SLAETS, Leen; SOMERS, Veerle; THOELEN, Ronald
- Source
- Subject
- Electrical impedance
microfluidics
ELISA
immunoassay
lab-on-card
label-free
- Language
- English
A high throughput solid-phase enzyme immunoassay (EIA) platform based on lab-on-card (LOC) microfluidics is proposed. In addition, a first step towards label-free detection of immunosorbent assays based on Electrical Impedance Spectroscopy (EIS) is made. The reported LOCs are developed by alternately stacking PET layers and adhesive layers. LOCs can be equipped with aluminium interdigitated electrodes and coated with polystyrene to perform EIS. Furthermore, the end product of a colorimetric ELISA test was flushed into a LOC and subsequently the optical density was measured. The preliminary tests provide a stable base for the label-free EIA based on EIS. This work is funded by the HEALTHY AGING project which is carried out under Interreg V-A Euregio Meuse - Rhine and is supported by the European Union and The European Regional Development Fund and with financial support of province of Limburg - Belgium.