The aim of the present study was to explore the effects of dicyandiamide(DCD)and surfactant on the form and content of nitrogen in digested slurry, using a laboratory simulation experiment with different combinations of application of the treatments. Six treatments were used:single application of digested slurry, digested slurry+8%DCD, digested slurry+16%DCD, digested slurry+surfactant, digested slurry+8%DCD+surfactant, and digested slurry+16%DCD+sufactant. The total nitrogen(TN), total Kjeldahl nitrogen(TKN), ammonium nitrogen(NH4+-N), and nitrate nitrogen(NO3--N)content of digested slurry were measured at 10, 30 min, and 1, 3, 10, 24, 48 h. The results revealed that, in the digested slurry+8%DCD and digested slurry+16%DCD treatments, the concentrations of NH4+-N, TKN, and NO3--N increased by 0.74%~8.96%, 24.67~59.70 mg·L-1, 13.42~145.88 times, respectively, and the concentrations of all three increased with the quantity of DCD. The addition of surfactant had little effect on the form and concentration of nitrogen in digested slurry.