Development and validation of RP HPLC method for determination of betamethasone dipropionate in gingival crevicular fluid
- Resource Type
- Authors
- Liljana Bogdanovska; Rumenka Petkovska; Aneta Dimitrovska; Mirjana Popovska
- Source
- Acta Pharmaceutica
Volume 63
Issue 3
- Subject
- Pharmacology
Chromatography
Chemistry
Calibration curve
Chemistry, Pharmaceutical
Pharmaceutical Science
Betamethasone dipropionate
Gingival Crevicular Fluid
General Medicine
medicine.disease
Betamethasone
High-performance liquid chromatography
Chronic periodontitis
Matrix (chemical analysis)
Crevicular fluid
medicine
Humans
Sample preparation
betamethasone dipropionate
gingival crevicular fluid
HPLC
periodontal disease
Chromatography, High Pressure Liquid
medicine.drug
- Language
- English
- ISSN
- 1846-9558
1330-0075
A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the concentration range of 0.10-2.00 μg mL-1 (R2 = 0.9971). RSD values of intra- and inter-day precision ranged 2.2-4.5 and 1.6-5.7 %, while accuracy values were higher than 96.6 and 97.0 %, respectively. The described method can be successfully applied for determination of betamethasone concentrations in GCF obtained from patients with chronic periodontitis after local treatment with BDP cream 0.5 mg g-1.