Additional file 2. Figure S2. Multiple alignments of selected protein families. A. Selected representatives taken from multiple alignment of precursors from cluster 2. Conserved residues in leader region are colored green, double glycine motif���blue, amino acids involved in ester and amide bonds formation���red; Underlined residues correspond to motifs of groups 8, 9 and 11 described in Lee et al. [24]. In addition, consensus sequences from Salinispora and cluster 199 aligned manually to show similarity within leader region. Abbreviations: gr8, gr9 and gr11���sequences with identified motifs of respective groups of core peptides delineated in Lee et al. [24]; ���no������sequences with no identified motifs delineated in Lee et al. [24]. B. Selected representatives taken from multiple alignment of precursors from cluster 13. Coloring is the same as in the Supplementary Figure 2A. Abbreviations: gr3, gr4, gr5 and gr6���sequences with identified motifs of respective groups of core peptides delineated in Lee et al. [24], respective motifs are underlined; ���no������sequences with no identified motifs delineated in Lee et al. [24]. The regions with a single core motif are shown by the red outline. C. Multiple alignment of LPL family of proteins. Alignments were colored using http://www.bioinformatics.org/sms2/color_align_cons.html server with default amino acid groups with 50% consensus. D. Multiple alignment of Cluster 23 and homologs. Alignments were colored using http://www.bioinformatics.org/sms2/color_align_cons.html server with default amino acid groups with 70% consensus. Residues within signal peptide region are colored cyan. Positions with conserved histidine, aspartate and asparagine marked by red letters H, D and R above the alignment. E. Multiple sequence alignment of chryseoviridin-like precursors. Alignments were colored using http://www.bioinformatics.org/sms2/color_align_cons.html server with default amino acid groups with 100% consensus. Amino acids shown experimentally to be involved in formation of lactam linkages are mapped on the CdnA3 sequence (Zhao et al., 2021).