Additional file 2: Figure S2. Increasing yields predicted by (a) mean oocyst densities (yellow line) correspond with increasing uncertainty (yellow shaded area). (b) In groups with higher mean oocyst densities (e.g., 45), estimating the contribution of time may be difficult because of the possibility of pooling individuals with heavily infected midgut (~ 50 oocysts, left pane) consisting of some oocysts that have contributed sporozoites already (arrows) and some still in the process of doing so (arrowheads), with another individual with low infected midgut (4 oocysts, right pane) where the entire contingent of sporozoites have been released (arrowheads). Images were taken at 400 × magnification at 26 days post-blood meal, from individuals whose salivary glands were combined into the same pool.